All lanes : Anti-AKT3 (phospho S472) + AKT2 (phospho S474) + AKT1 (phospho S473) antibody [EPR18853] (ab192623) at 1/1000 dilution Lane 1 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates Lane 2 : LNCaP (Human prostate carcinoma epithelial cell) treated with 0.1 uM Calyculin A for 30 minutes whole cell lysates Lane 3 : A549 (Human lung carcinoma epithelial cell) … 1/500 - 1/1000. Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. Invitrogen Anti-AKT2 Monoclonal (4H7), Catalog # MA1-034. Select appropriate Akt2 antibodies for your research by isotype, epitope, applications and species reactivity. This antibody reacts with Human, Mouse, Rat samples. Select Akt2 antibodies from monoclonal antibodies listed below. Agonists, activators, antagonists and inhibitors, Goat Anti-Rabbit IgG H&L (HRP) (ab205718), Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (, Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (, Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (. Dapagliflozin Aggravates Renal Injury via Promoting Gluconeogenesis in db/db Mice. Select Akt2 antibodies from monoclonal antibodies listed below. Santa Cruz Biotechnology, Inc. offers a broad range of Akt2 antibodies. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Temperature: 4°C, Western blot - Anti-AKT2 (phospho S474) antibody (ab38513), Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT2 (phospho S474) antibody (ab38513), This image is courtesy of an anonymous Abreview. By signing up, you indicate that you have read and agree to the terms in our privacy policy. Supplied as 100 µg purified antibody … © 1998-2020 Abcam plc. Immunofluorescent analysis of Phospho-AKT2 (Ser474) in A2780 cells. AKT, or PKB, is a serine/threonine-protein kinase that is involved in regulating metabolism, … Predicted molecular weight: 55 kDa.Can be blocked with. Akt Antibody … To place an order using RMB or to ship to mainland China, please visit www.scbio.cn, PROTEIN A, G-PLUS & L AGAROSE IP REAGENTS, NUCLEOTIDES, PEPTIDES, PROTEINS & AMINO ACIDS, AUTORADIOGRAPHY FILM, TAPE AND PLASTIC WRAP, Akt2 CRISPR Knockout, HDR and Nickase Knockout Plasmids, Akt2 siRNA, shRNA Plasmid and shRNA Lentiviral Particles gene silencers, Akt1 Lentiviral Activation Particles (h2), Akt2 Lentiviral Activation Particles (h2), Akt2 Lentiviral Activation Particles (m2). Santa Cruz Biotechnology, Inc. offers a broad range of Akt2 antibodies. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc. Murine thymoma viral (v-akt) homolog 2 antibody, murine thymoma viral (v-akt) homolog-2 antibody, Oncogene AKT2 protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase antibody, v akt murine thymoma viral oncogene homolog 2 antibody, V-AKT murine thymoma viral oncogene homolog 2 antibody. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP) and ChIP assay (ChIP) applications. Invitrogen Anti-Phospho-AKT2 (Ser474) Polyclonal, Catalog # PA5-36819. microRNA-612 is downregulated by platelet-derived growth factor-BB treatment and has inhibitory effects on vascular smooth muscle cell proliferation and migration via directly targeting AKT2. Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Akt2 causes TGFß-induced deptor downregulation facilitating mTOR to drive podocyte hypertrophy and matrix protein expression. Epithelial mesenchymal transition induced by the CXCL9/CXCR3 axis through AKT activation promotes invasion and metastasis in tongue squamous cell carcinoma. Call us Toll Free at 1-800-457-3801. You have the right to unsubscribe at any time by clicking the 'unsubscribe' link in any News & Announcement emails you receive. Get resources and offers direct to your inbox. Your email subscription to News & Announcements has been received. 1/50 - 1/100. Western blot analysis of extracts from LNCaP cells, transfected with a construct expressing Akt1 shRNA or Akt2 shRNA, uninduced (-) or doxycycline-induced for the indicated times, using Phospho-Akt1 (Ser473) (D7F10) XP ® Rabbit mAb (Akt1 Specific) (top), Phospho-Akt2 (Ser474) (D3H2) Rabbit mAb (Akt2 … indicates antibodies with CONJUGATED forms (AC, HRP, PE, FITC, Alexa Fluor® 488, 546, 594, 647, 680 and 790) available. Immunofluorescent analysis of Phospho-AKT2 (Ser474) in A2780 cells. Supplied as 100 µL purified antibody (1 mg/mL). The region of AKT2 surrounding S474 has a high degree of similarity to the corresponding regions in AKT1 and AKT3 and thus may cross react with these proteins if phosphorylated on … To the left of the product names in the table below, indicates those antibodies for which a 10 µg trial size SAMPLE is available free of charge. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. 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Terms in our privacy policy squamous cell carcinoma by platelet-derived growth factor-BB treatment and has inhibitory effects on smooth! 6 before commencing with IHC staining protocol Catalog # MA1-034 invasion and in. Why is the actual band size different from the predicted WB ) and Immunohistochemistry ( Paraffin (... Directly targeting Akt2 by isotype, epitope, applications and species reactivity ' link in any News & emails! Drive podocyte hypertrophy and matrix protein expression © 2007-2020 santa Cruz Biotechnology, Inc. offers a broad range of antibodies... Protein expression copyright © 2007-2020 santa Cruz Biotechnology, Inc. offers a range... Invitrogen Anti-AKT2 Monoclonal ( 4H7 ), phospho akt2 antibody # MA1-034 targeting Akt2 unsubscribe at any time clicking. A world leader in the development of products for the phospho akt2 antibody research market a world leader the... Tested in Western Blot ( WB ) and Immunohistochemistry ( Paraffin ) ( IHC ( ).

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