Measurement of Log D by the shake flask method as a measure of lipophilicity Service details. Now to your questions: * How much do you “shake” the flask? shake flask experiment and some of these alternatives have been recently reviewed, and the shake flask derived data are still viewed as the gold standard in terms of quality but with signifi-cant drawbacks in terms of sample throughput capacity.12 the method that has been developed is a miniaturized and automated shake flask log D 7.4 This commonly used shake flask protocol is based on the use of Millipore Multiscreen solubility filter plates or centrifugation followed by UV-Vis quantitation of dissolved compounds. Note: Make sure that the shaker flask does not have baffles (i.e., the indents at the bottom of the flask designed to provide agitation), because they ruin the shaking rhythm. Several procedures based on the shake-flask method and designed to require a minimum amount of drug for octanol-water partition coefficient determination have been established and developed. Shake flask-type. Cyprotex deliver consistent, high quality data with cost-efficiency that comes from a highly automated approach. When the cells are ready for passaging (i.e., log-phase growth before they reach confluency), remove the flask from the incubator and take a small sample from the culture flask using a sterile pipette. Several procedures based on the shake-flask method and designed to require a minimum amount of drug for octanol-water partition coefficient determination have been established and developed. Use the Log D shake flask assay to determine the lipophilicity of your compound. Measurements are usually performed by the shakeflask method with UV-Vis or LC-MS detection. The aqueous phase should be sampled by a procedure that minimizes the risk of including traces of n-octanol. The procedures have been validated by a 28 substance set with a lipophilicity range from -2.0 to 4.5 (logD7.4 … A new method for determination of distribution coefficient of drugs azathioprine, 6-mercaptopurine and 6-thioguanine and nutrient folic acid used in the treatment of inflammatory bowel disease based on a miniaturized shake-flask and HPLC/DAD was developed. Shake flask solubility determinations are quite straightforward but there are a few mistakes that are easy to make for a beginner. The classical and most reliable method of log P determination is the shake-flask method, which consists of dissolving some of the solute in question in a volume of octanol and water, then measuring the concentration of the solute in each solvent. This can be done using a syringe with a removable needle. Log D 7.4 shake flask is one of Cyprotex's in vitro ADME screening services. Abstract.
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